Downregulated ALKBH5 contributes to myocardial ischemia/reperfusion injury by increasing m⁶A modification of Trio mRNA.

ABSTRACT:

Background

The modification of N⁶-methyladenosine (m⁶A) is a dynamic and reversible course that might play a role in cardiovascular disease. However, the mechanisms of m⁶A modification in myocardial ischemia/reperfusion injury (MIRI) remain unclear.

Methods

A mouse model of MIRI and a cell model of oxygen-glucose deprivation/reperfusion (OGD/R) HL-1 cells were employed. In an in vivo study, the total RNA m⁶A modification levels were determined by dot blot, and the key genes related to m⁶A modification were screened by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. In an in vitro study, the effects of AlkB homolog 5 (ALKBH5), an RNA demethylase, on cell proliferation, cell injur

SUBMITTER: Li J 

PROVIDER: S-EPMC9073777 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature