Uptake, functionality, and re-release of extracellular vesicle-encapsulated cargo.

ABSTRACT: Extracellular vesicles (EVs) are membrane-encapsulated particles that carry genetically active and protein/lipid cargo that can affect the function of the recipient cell. A number of studies have described the effect of these vesicles on recipient cells and demonstrated their promise as therapeutic delivery vectors. Here we demonstrate functional delivery of EV-encapsulated RNA and protein cargo through use of luminescence and fluorescence reporters by combining organelle-targeted nanoluciferase with fluorescent proteins. We highlight a mechanism by which cells retain internalized cargo in the endosomal compartment for days, usually leading to content degradation. We also identify a mode through which recipient cells re-release internalized EVs intact after uptake. Highlighting these diffe

SUBMITTER: O'Brien K 

PROVIDER: S-EPMC9074118 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature