Clickable poly-l-lysine for the formation of biorecognition surfaces.
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ABSTRACT:
SUBMITTER: Di Iorio D
PROVIDER: S-EPMC9074408 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Publications
Clickable poly-l-lysine for the formation of biorecognition surfaces.
Di Iorio Daniele D Marti Almudena A Koeman Sander S Huskens Jurriaan J
RSC advances 20191001 61
Biomolecules are immobilized onto surfaces employing the fast and stable adsorption of poly-l-lysine (PLL) polymers and the versatile copper-free click chemistry reactions. This method provides the combined advantages of versatile surface adsorption with density control using polyelectrolytes and of the covalent and orthogonal immobilization of biomolecules with higher reaction rates and improved yields of click chemistry. Using DNA attachment as a proof of concept, control over the DNA probe de ...[more]
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