Kinetic and spectroscopic responses of pH-sensitive nanoparticles: influence of the silica matrix.

ABSTRACT: Intracellular pH sensing with fluorescent nanoparticles is an emerging topic as pH plays several roles in physiology and pathologic processes. Here, nanoparticle-sized pH sensors (diameter far below 50 nm) for fluorescence imaging have been described. Consequently, a fluorescent derivative of pH-sensitive hydroxypyrene with pK _a = 6.1 was synthesized and subsequently embedded in core and core-shell silica nanoparticles via a modified Stöber process. The detailed fluorescence spectroscopic characterization of the produced nanoparticles was carried out for retrieving information about the environment within the nanoparticle core. Several steady-state and time-resolved fluorescence spectroscopic methods hint to the screening of the probe molecule from the solvent, but

SUBMITTER: Clasen A 

PROVIDER: S-EPMC9074731 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature