Preparative separation of seven phenolic acids from Xanthii Fructus using pH-zone-refining counter-current chromatography combined with semi-preparative high performance liquid chromatography.

ABSTRACT: Phenolic acids represented by caffeoylquinic acids in Xanthii Fructus have various pharmacological activities such as anti-inflammatory, anti-nociceptive, anti-oxidative and anti-allergic effects. In this study, pH-zone-refining counter-current chromatography was successfully applied in the segmentation of crude samples and further separation of phenolic acids from Xanthii Fructus. We initially segmented 1.6 g of the crude sample to yield three sample fractions using a two-phase solvent system composed of EtOAc-ACN-H₂O (4 : 1 : 5, v/v/v) with 10 mM TFA added to the organic phase as the stationary phase and 10 mM NH₃·H₂O added to the aqueous phase as the mobile phase. The first fraction was separated using EtOAc-H₂O (1 : 1, v/v) (10 mM TFA was add

SUBMITTER: Ma T 

PROVIDER: S-EPMC9075151 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature