A fluorescence polarization immunoassay for detection of thiacloprid in environmental and agricultural samples.

ABSTRACT: As a widely used neonicotinoid insecticide, thiacloprid has been observed to pose a risk to honeybees and the endocrine system of mammals. So a detection method with high sensitivity, simple operation and high throughput is required. Based on this consideration, we prepared an anti-thiacloprid monoclonal antibody (mAb, C9) and developed a fluorescence polarization immunoassay (FPIA) for the detection of thiacloprid. After optimizing the length of spacer and reaction conditions, the 50% inhibition concentration (IC₅₀), limit of detection (LOD) and linear range (IC₂₀ ∼ IC₈₀) of the FPIA are 15.34 ng mL^-1, 2.43 ng mL^-1 and 3.10-65.7 ng mL^-1, respectively. Meanwhile, FPIA just requires 12 min to detect the pesticide with simple

SUBMITTER: Ding Y 

PROVIDER: S-EPMC9075169 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature