An amphiphilic-ligand-modified gold nanoflower probe for enhancing the stability of lateral flow immunoassays in dried distillers grains.

ABSTRACT: An amphiphilic ligand-capped gold nanoflower (AuNF) was proposed as a novel lateral flow immunoassay (LFA) reporter for zearalenone (ZEN) detection in distillers dried grains solubles (DDGS). The amphiphilic ligand consists of a thiol-terminated hydrophobic alkane chain, a tetra (ethylene glycol) unit, and a terminal carboxyl group. The novel AuNF probe (N-AuNF-Abs) was prepared by coupling the amino group of anti-ZEN antibodies with the AuNF carboxyl group via an amido covalent linkage. For comparison, a traditional AuNF probe (Tr-AuNF-Abs) was prepared by labeling antibodies on the surface of citrate capped AuNFs via an electrostatic adsorption method. The detection performance of the two probes in LFA was systematically investigated, including the half maximal inhibitory c

SUBMITTER: Ma T 

PROVIDER: S-EPMC9075177 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature