Profiling senescent cells in human brains reveals neurons with CDKN2D/p19 and tau neuropathology.

ABSTRACT: Senescent cells contribute to pathology and dysfunction in animal models¹. Their sparse distribution and heterogenous phenotype have presented challenges for detecting them in human tissues. We developed a senescence eigengene approach to identify these rare cells within large, diverse populations of postmortem human brain cells. Eigengenes are useful when no single gene reliably captures a phenotype, like senescence; they also help to reduce noise, which is important in large transcriptomic datasets where subtle signals from low-expressing genes can be lost. Each of our eigengenes detected ~2% senescent cells from a population of ~140,000 single nuclei derived from 76 postmortem human brains with various levels of Alzheimer's disease (AD) pathology. More than 97% of the senesce

SUBMITTER: Dehkordi SK 

PROVIDER: S-EPMC9075501 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature