Single-cell transcriptome highlights a multilayer regulatory network on an invasive trajectory within colorectal cancer progression.

ABSTRACT:

Purpose

Colorectal cancer (CRC) is one of the most common and fatal gastrointestinal malignancies, in which cancer stem cells (CSCs) were identified to enable tumor heterogeneity and initiate tumor formation. However, the process from CSCs to invasion cells is unconfirmed.

Methods

Several bioinformatics methods, including clustering, pseudotime analysis, gene set variation analysis and gene ontology enrichment, were used to construct a path of gradual transformation of CSCs to invasive cells, called "stem-to-invasion path". A large amount of signaling interactions were collated to build the multilayer regulatory network. Kaplan-Meier curve and time-dependent ROC method were applied to reveal prognostic values.

Results

We validated the heterogeneity of cells in the tum

SUBMITTER: Zhou T 

PROVIDER: S-EPMC9075720 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature