Dissipative biocatalytic cascades and gated transient biocatalytic cascades driven by nucleic acid networks.

ABSTRACT: Living systems consist of complex transient cellular networks guiding structural, catalytic, and switchable functions driven by auxiliary triggers, such as chemical or light energy inputs. We introduce two different transient, dissipative, biocatalytic cascades, the coupled glucose oxidase (GOx)/horseradish peroxidase (HRP) glucose-driven oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS^2-) to the radical anion (ABTS^•-) and the lactate dehydrogenase (LDH)/nicotinamide adenine dinucleotide (NAD⁺) lactate-driven reduction of NAD⁺ to NADH. The transient biocatalytic systems are driven by nucleic acid reaction modules using a nucleic acid fuel strand L₁' and a nicking enzyme, Nt.BbvCI, as fuel-degrading catalyst, le

SUBMITTER: Ouyang Y 

PROVIDER: S-EPMC9075803 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature