NAT10-mediated mRNA N4-acetylcytidine modification promotes bladder cancer progression.

ABSTRACT:

Background

Dysregulation of the epitranscriptome causes abnormal expression of oncogenes in the tumorigenic process. Previous studies have shown that NAT10 can regulate mRNA translation efficiency through RNA acetylation. However, the role of NAT10-mediated acetylation modification in bladder cancer remains elusive.

Methods

The clinical value of NAT10 was estimated according to NAT10 expression pattern based on TCGA data set and the tumor tissue array. Acetylated RNA immunoprecipitation sequencing was utilized to explore the role of NAT10 in mRNA ac4C modification. Translation efficiency and mRNA stability assay were applied to study the effect of NAT10-deletion on target genes. The nude mouse model and genetically engineered mice were conducted to further verify the charact

SUBMITTER: Wang G 

PROVIDER: S-EPMC9076013 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature