Single-cell atlas of peripheral blood mononuclear cells from pregnant women.

ABSTRACT:

Background

During pregnancy, mother-child interactions trigger a variety of subtle changes in the maternal body, which may be reflected in the status of peripheral blood mononuclear cells (PBMCs). Although these cells are easy to access and monitor, a PBMC atlas for pregnant women has not yet been constructed.

Methods

We applied single-cell RNA sequencing (scRNA-seq) to profile 198,356 PBMCs derived from 136 pregnant women (gestation weeks 6 to 40) and a control cohort. We also used scRNA-seq data to establish a transcriptomic clock and thereby predicted the gestational age of normal pregnancy.

Results

We identified reconfiguration of the peripheral immune cell phenotype during pregnancy, including interferon-stimulated gene upregulation, activation of RNA splicing-re

SUBMITTER: Chen D 

PROVIDER: S-EPMC9076016 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature