Analysis of antisense oligonucleotides with the use of ionic liquids as mobile phase modifiers.

ABSTRACT: The main goal of this study was the investigation of the impact of several ionic liquids, commonly used as free silanol suppressors, on the retention and separation of phosphorothioate oligonucleotides. Three various stationary phases (octadecyl, octadecyl with embedded polar groups and pentafluorophenyl) as well as ionic liquids with the concentration range of 0.1-7 mM were used for this purpose. The results obtained during this study showed that the increase in concentration of ionic liquids results in increasing retention of the oligonucleotides. Such an effect was observed regardless of the stationary phase used. Moreover, elongation of the alkyl chain in the structure of ionic liquids caused an increase of antisense oligonucleotide retention factors. The results obtained during retent

SUBMITTER: Kaczmarkiewicz A 

PROVIDER: S-EPMC9076077 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature