RNA Demethylase ALKBH5 Prevents Lung Cancer Progression by Regulating EMT and Stemness via Regulating p53.

ABSTRACT:

Background

Although N6-methyladenosine (m⁶A) RNA methylation is the most abundant reversible methylation of mRNA, which plays a critical role in regulating cancer processing, few studies have examined the role of m⁶A in nonsmall-cell lung cancer-derived cancer stem-like cells (CSCs).

Methods

CSCs were enriched by culturing NSCLC cells in a serum-free medium, and stem factors, including CD24, CD44, ALDH1, Nanog, Oct4, and Sox2 were detected by Western blot. ALKBH5 expression was measured by employing a tissue array. Global m⁶A methylation was measured after ALKBH5 knockdown. Malignances of CSCs were detected by performing CCK-8 assay, invasion assay, cell cycle analysis, and tumor formation in vitro and in vivo.

Results

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SUBMITTER: Liu X 

PROVIDER: S-EPMC9076132 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature