Detection of carboxylesterase by a novel hydrosoluble near-infrared fluorescence probe.

ABSTRACT: A novel hydrosoluble near-infrared fluorescence off-on probe has been developed for detecting carboxylesterase activity. The probe was designed by introducing (4-acetoxybenzyl)oxy as a quenching and recognizing moiety to the decomposed product of IR-783, which exhibits excellent near-infrared fluorescence features and good water solubility. The responding mechanism of novel probe 1 to carboxylesterase was investigated. It would lead to the cleavage of the carboxylic ester bond by carboxylesterase catalyze the spontaneous hydrolysis of the probe, resulting in the release of a near-infrared fluorophore. This behaviour leads to the development of a simple and sensitive fluorescent method for assaying carboxylesterase activity, with a detection limit of 3.4 × 10^-3 U mL^-1.

SUBMITTER: Li M 

PROVIDER: S-EPMC9076276 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature