Dataset Information

Co²⁺ detection, cell imaging, and temperature sensing based on excitation-independent green-fluorescent N-doped carbon dots.

ABSTRACT: Green-fluorescent N-doped carbon dots (N-CDs) have been successfully fabricated using hydrothermal treatment of tyrosine and urea. The N-CDs obtained showed excitation-independent emission, superior stability and strong photoluminescence with a quantum yield of ca. 9.8%. Based on these striking behaviors, the as-prepared N-CDs have been utilized in Co²⁺ detection and temperature sensing. Due to an inner filter effect, the N-CDs obtained were dramatically quenched by Co²⁺ with linear ranges of 0.1 μM-10 μM, 25 μM-275 μM and 300 μM-400 μM, and they had a detection limit of 0.15 μM. The use of the as-prepared N-CDs has been extended to visualize Co²⁺ fluctuations in living cells. Additionally, the N-CDs obtained have also been applied for use as a temperature sensor with a linear range of 25-80 °C.

SUBMITTER: Shi L

PROVIDER: S-EPMC9076455 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Co2+ detection, cell imaging, and temperature sensing based on excitation-independent green-fluorescent N-doped carbon dots.

Shi Lihong L Chang Dan D Zhang Guomei G Zhang Caihong C Zhang Yan Y Dong Chuan C Chu Lanling L Shuang Shaomin S

RSC advances 20191216 70

Green-fluorescent N-doped carbon dots (N-CDs) have been successfully fabricated using hydrothermal treatment of tyrosine and urea. The N-CDs obtained showed excitation-independent emission, superior stability and strong photoluminescence with a quantum yield of ca. 9.8%. Based on these striking behaviors, the as-prepared N-CDs have been utilized in Co2+ detection and temperature sensing. Due to an inner filter effect, the N-CDs obtained were dramatically quenched by Co2+</ ...[more]

PMID: 35540082

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Project description:Fluorescent carbon dots (CDs) have been identified as potential nanosensors and attracted tremendous research interests in wide areas including anti-counterfeiting, environmental and biological sensing and imaging in considering of the attractive optical properties. In this work, we present a CDs based fluorescent sensor from polyvinylpyrrolidone, citric acid, and methionine as precursors by hydrothermal approach. The selective quantifying of Fe3+ and ascorbic acid (AA) are based on the fluorescent on-off-on process, in which the fluorescent quenching is induced by the coordination of the Fe3+ on the surface of the CDs, while the fluorescence recovery is mainly attributed to redox reaction between Fe3+ and AA, breaking the coordination and bringing the fluorescence back. Inspired by the good water solubility and biocompatibility, significant photostability, superior photobleaching resistance as well as high selectivity, sensitivity, and interference immunity, which are constructed mainly from the N,S-doping and methionine surface functionalization, the CDs have not only been employed as fluorescence ink in multiple anti-counterfeiting printing and confidential document writing or transmitting, but also been developed as promising fluorescence sensors in solution and solid by CDs doped test strips and hydrogels for effectively monitoring and removing of Fe3+ and AA in environmental aqueous solution. The CDs have been also implemented as effective diagnostic candidates for imaging and tracking of Fe3+ and AA in living cells, accelerating the understanding of their function and importance in related biological processes for the prevention and treatment specific diseases.Electronic supplementary materialSupplementary material (fluorescence spectra: UV and Xe irradiation, TG, thermo stability, ionic strength, relationship between fluorescence responses at different concentrations of Fe3+ and AA, reaction time-dependent fluorescent responses; XPS spectra of CDs + Fe3+ and Fe3+@CDs + AA; structural characterization; equations about fluorescence lifetime, quantum yield and LOD; comparison of the CDs for the detection of Fe3+ and AA with reported methods; detection of Fe3+ and AA in real samples; absorption of Fe3+ in environmental samples and MTT assay results) is available in the online version of this article at 10.1007/s12274-022-5107-7.

Dataset Information

Co²⁺ detection, cell imaging, and temperature sensing based on excitation-independent green-fluorescent N-doped carbon dots.

Publications

Co<sup>2+</sup> detection, cell imaging, and temperature sensing based on excitation-independent green-fluorescent N-doped carbon dots.

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