Proof of concept web application for understanding the energetic basis of oligonucleotide unfolding.

ABSTRACT: Measuring and quantifying thermodynamic parameters that determine both the stability of and interactions between biological macromolecules are an essential and necessary complement to structural studies. Although basic thermodynamic parameters for an observed process can be readily obtained, the data interpretation is often slow and analysis quality can be extremely variable. We have started to develop a web application that will help users to perform thermodynamic characterizations of oligonucleotide unfolding. The application can perform global fitting of calorimetric and spectroscopic data, and uses a three-state equilibrium model to obtain thermodynamic parameters for each transition step - namely, the Gibbs energy, the enthalpy, and the heat capacity. In addition, the application can

SUBMITTER: Prislan I 

PROVIDER: S-EPMC9076490 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature