HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophages.

ABSTRACT: Macrophages are key elements of the innate immune system. Their HIV-1 infection is a complex process that involves multiple interacting factors and various steps and is further altered by exposure of infected cells to methamphetamine (Meth), a common drug of abuse in people living with HIV. This is reflected by dynamic changes in the intracellular and secreted proteomes of these cells. Quantification of these changes poses a challenge for experimental design and associated analytics. In this study, we measured the effect of Meth on expression of intracellular and secreted galectins-1, -3, and -9 in HIV-1 infected human monocyte-derived macrophages (hMDM) using SWATH-MS, which was further followed by MRM targeted mass spectrometry validation. Cells were exposed to Meth either prior to or af

SUBMITTER: Grabowska K 

PROVIDER: S-EPMC9076712 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature