Population genomics for symbiotic anthozoans: can reduced representation approaches be used for taxa without reference genomes?

ABSTRACT: Population genetic studies of symbiotic anthozoans have been historically challenging because their endosymbioses with dinoflagellates have impeded marker development. Genomic approaches like reduced representation sequencing alleviate marker development issues but produce anonymous loci, and without a reference genome, it is unknown which organism is contributing to the observed patterns. Alternative methods such as bait-capture sequencing targeting Ultra-Conserved Elements are now possible but costly. Thus, RADseq remains attractive, but how useful are these methods for symbiotic anthozoan taxa without a reference genome to separate anthozoan from algal sequences? We explore this through a case-study using a double-digest RADseq dataset for the sea anemone Bartholomea annulata. We assemb

SUBMITTER: Titus BM 

PROVIDER: S-EPMC9076904 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature