Ontology highlight
ABSTRACT:
SUBMITTER: Kong G
PROVIDER: S-EPMC9076968 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Kong Guiping G Zhou Luming L Freiwald Anja A Shkura Kirill K Di Giovanni Simone S
STAR protocols 20220205 1
The study of neuronal signaling <i>ex vivo</i> requires the identification of the proteins that are represented within the neuronal axoplasm. Here, we describe a detailed protocol to isolate the axoplasm of peripheral and central axonal branches of sciatic dorsal root ganglia neurons in mice. The axoplasm is separated by 2D gel and digestion followed by proteomics analysis with MS/MS-LC. This protocol can be applied to dissect the axoplasmic protein expression signatures before and after a sciat ...[more]