Tumor angiogenesis targeting and imaging using gold nanoparticle probe with directly conjugated cyclic NGR.

ABSTRACT: Angiogenesis is a vital process for the growth and metastasis of malignant tumor. Visualization of tumor angiogenesis is thus of great importance in the evaluation of biologic aggressiveness as well as monitoring of the response to anti-angiogenic therapy. Herein, we developed a probe based on gold nanoparticles (GNPs) directly surface-functionalized with a tumor-homing cyclized asparagine-glycine-arginine peptide (SH-cNGR) and carboxylpoly(ethylene glycol)thiol (SH-PEG-COOH) via Au-S bonds. The obtained GNPs-PEG@cNGR probe was used to target the aminopeptidase-N (APN/CD13), which overexpressed in the endothelium of tumor angiogenesis. The CD13 binding affinities of the peptides were assessed by a receptor binding assay based on HUVEC and HepG2 cell (e.g. fluorescence imaging

SUBMITTER: Wu M 

PROVIDER: S-EPMC9077115 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature