METTL3-mediated m⁶A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.

ABSTRACT: Epigenetic changes are present in many physiological and pathological processes. The N⁶-methyladenosine (m6A) modification is the most common modification in eukaryotic mRNA. However, the role of m6A modification in diabetic nephropathy (DN) remains elusive. Here, we found that m6A modification was significantly upregulated in the kidney of type 1 and type 2 diabetic mice, which was caused by elevated levels of METTL3. Moreover, METTL3 is increased in podocyte of renal biopsy from patients with DN, which is related to renal damage. METTL3 knockout significantly reduced the inflammation and apoptosis in high glucose (HG)-stimulated podocytes, while its overexpression significantly aggravated these responses in vitro. Podocyte-conditional knockout METTL3 significantly alleviated p

SUBMITTER: Jiang L 

PROVIDER: S-EPMC9077313 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature