RNA m⁶A methylation regulates dissemination of cancer cells by modulating expression and membrane localization of β-catenin.

ABSTRACT: N6-methyladenosine (m⁶A) methylation, which is modified by the METTL3/METTL14 complex, is a dominant internal modification in mammalian RNA and tightly linked to cancer progression. Here we reveal that METTL3-promoted cell migration, invasion, and epithelial-to-mesenchymal transition (EMT) are associated with expression and membrane localization of β-catenin (encoded by CTNNB1), as opposed to Wnt signaling activation in various types of cancer cells, including cervical, lung, and liver cancer. Specifically, METTL3 regulates the transcription, mRNA decay, translation, and subcellular localization of β-catenin. For CTNNB1 expression, METTL3 indirectly suppresses CTNNB1 transcription by stabilizing its transcription suppressor E2F1 mRNA； deposition of 5'UTR m⁶A in CTNNB1

SUBMITTER: Li J 

PROVIDER: S-EPMC9077323 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature