Pitch-tunable pillar arrays for high-throughput culture and immunohistological analysis of tumor spheroids.

ABSTRACT: Tumor spheroids are multicellular, three-dimensional (3D) cell culture models closely mimicking the microenvironments of human tumors in vivo, thereby providing enhanced predictability, clinical relevancy of drug efficacy and the mechanism of action. Conventional confocal microscopic imaging remains inappropriate for immunohistological analysis due to current technical limits in immunostaining using antibodies and imaging cells grown in 3D multicellular contexts. Preparation of microsections of these spheroids represents a best alternative, yet their sub-millimeter size and fragility make it less practical for high-throughput screening. To address these problems, we developed a pitch-tunable 5 × 5 mini-pillar array chip for culturing and sectioning tumor spheroids in a high throughp

SUBMITTER: Lee DW 

PROVIDER: S-EPMC9077751 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature