A novel and sensitive DNA methylation marker for the urine-based liquid biopsies to detect bladder cancer.

ABSTRACT:

Background

Better prognostic outcome is closely correlated with early detection of bladder cancer. Current non-invasive urianalysis relies on simultaneously testing multiple methylation markers to achieve relatively high accuracy. Therefore, we have developed an easy-to-use, convenient, and accurate single-target urine-based DNA methylation test for the malignancy.

Methods

By analyzing TCGA data, 344 candidate markers with 424 primer pairs and probe sets synthesized were systematically screened in cancer cell lines, paired tissue specimens, and urine sediments from bladder cancer patients and normal controls. The identified marker was further validated in large case-control cohorts. Wilcoxon rank sum tests and c2 tests were performed to compare methylation levels between cas

SUBMITTER: Deng L 

PROVIDER: S-EPMC9077853 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature