Construction and application of star polycation nanocarrier-based microRNA delivery system in Arabidopsis and maize.

ABSTRACT:

Background

MicroRNA (miRNA) plays vital roles in the regulation of both plant architecture and stress resistance through cleavage or translation inhibition of the target messenger RNAs (mRNAs). However, miRNA-induced gene silencing remains a major challenge in vivo due to the low delivery efficiency and instability of miRNA, thus an efficient and simple method is urgently needed for miRNA transformation. Previous researches have constructed a star polycation (SPc)-mediated transdermal double-stranded RNA (dsRNA) delivery system, achieving efficient dsRNA delivery and gene silencing in insect pests.

Results

Here, we tested SPc-based platform for direct delivery of double-stranded precursor miRNA (ds-MIRNA) into protoplasts and plants. The results showed that SPc could assembl

SUBMITTER: Yang J 

PROVIDER: S-EPMC9077854 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature