BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia.

ABSTRACT:

Background

The BIN1 locus contains the second-most significant genetic risk factor for late-onset Alzheimer's disease. BIN1 undergoes alternate splicing to generate tissue- and cell-type-specific BIN1 isoforms, which regulate membrane dynamics in a range of crucial cellular processes. Whilst the expression of BIN1 in the brain has been characterized in neurons and oligodendrocytes in detail, information regarding microglial BIN1 expression is mainly limited to large-scale transcriptomic and proteomic data. Notably, BIN1 protein expression and its functional roles in microglia, a cell type most relevant to Alzheimer's disease, have not been examined in depth.

Methods

Microglial BIN1 expression was analyzed by immunostaining mouse and human brain, as well as by immunoblot and

SUBMITTER: Sudwarts A 

PROVIDER: S-EPMC9077874 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature