Comparative analysis of novel and common reference genes in adult tissues of the mussel Mytilus galloprovincialis.

ABSTRACT:

Background

Real-time quantitative PCR is a widely used method for gene expression analyses in various organisms. Its accuracy mainly relies on the correct selection of reference genes. Any experimental plan involving real-time PCR needs to evaluate the characteristics of the samples to be examined and the relative stability of reference genes. Most studies in mollusks rely on reference genes commonly used in vertebrates.

Results

In this study, we focused on the transcriptome of the bivalve mollusk Mytilus galloprovincialis in physiological state to identify suitable reference genes in several adult tissues. Candidate genes with highly stable expression across 51 RNA-seq datasets from multiple tissues were selected through genome-wide bioinformatics analysis. This approach le

SUBMITTER: Salatiello F 

PROVIDER: S-EPMC9077915 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature