A light-responsive multienzyme complex combining cascade enzymes within a peptide-based matrix.

ABSTRACT: In this study, a light-responsive multienzyme complex (GOx&hemin@PepM) was developed by incorporating glucose oxidase (GOx) and hemin within a peptide-based matrix. An azobenzene group (Azo) was linked to the N-terminucs of glycine-phenylalanine-glycine tripeptide (GFG) to facilitate the formation of the supramolecular peptide-based matrix. Due to the proximity effect of GOx and hemin in the matrix as well as the biomimetic microenvironment of the peptide-based material, the transfer of intermediates can be enhanced and the catalytic activity of this multienzyme complex was greatly improved over free enzymes for catalyzing a cascade reaction. In addition, based on the light-responsive conformational switching of azobenzene between E and Z forms, the structure of the peptide-b

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9078227 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature