Metabolite identification of ursolic acid in mouse plasma and urine after oral administration by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry.

ABSTRACT: Ursolic acid (UA), a pentacyclic terpenoid carboxylic acid widely existing in various medicinal plants, has been reported to have multifarious biological activities such as anti-inflammatory, anticancer and antioxidant activities. In this paper, we analyzed the metabolic profile of UA in mice (including plasma and urine) by using ultra-high performance liquid chromatography (UPLC) coupled with a quadrupole time-of-flight (Q/TOF) method. Principal component analysis (PCA) was applied to differentiate the control and experimental groups. Potential biomarkers were filtered by using loading plots followed by further analysis with UPLC-Q/TOF-MS data. The results showed that 3 metabolites in plasma were identified as markers, one of which was UA and the others were UA epoxides, which belonged to

SUBMITTER: Hu X 

PROVIDER: S-EPMC9078307 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature