Sweeping-micelle to solvent stacking for the on-line preconcentration and determination of organic acids in Angelica sinensis by capillary electrophoresis.

ABSTRACT: A novel on-line two-step stacking preconcentration method by sweeping plus micelle to solvent stacking in capillary zone electrophoresis was developed for the simultaneous determination of three organic anions (vanillic acid, ferulic acid and cinnamic acid) in Angelica sinensis. Hexadimethrine bromide was used for electroosmotic flow reversal. The main experimental parameters that affected the separation and sensitivity were investigated and optimized. The best separation was achieved in 50 mM ammonium acetate (pH 12.0) containing 50% methanol (v/v) under a negative voltage of 20 kV. The micellar solution was a mixed solution comprised of 12 mM cetyltrimethyl ammonium bromide and 20 mM ammonium acetate. After injecting a short plug (0.5 psi, 30 s) of micellar solution, the sample wa

SUBMITTER: Yang X 

PROVIDER: S-EPMC9078526 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature