Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai.

ABSTRACT: Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended c

SUBMITTER: Izrael-Zivkovic L 

PROVIDER: S-EPMC9078880 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature