Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE.

ABSTRACT: Melibiose-derived AGE (MAGE) is an advanced glycation end-product formed in vitro in anhydrous conditions on proteins and protein-free amino acids during glycation with melibiose. Our previous studies revealed the presence of MAGE antigen in the human body and tissues of several other species, including muscles, fat, extracellular matrix, and blood. MAGE is also antigenic and induces generation of anti-MAGE antibody. The aim of this paper was to identify the proteins modified by MAGE present in human body fluids, such as serum, plasma, and peritoneal fluids. The protein-bound MAGE formed in vivo has been isolated from human blood using affinity chromatography on the resin with an immobilized anti-MAGE monoclonal antibody. Using mass spectrometry and immunochemistry it has been established

SUBMITTER: Gostomska-Pampuch K 

PROVIDER: S-EPMC9079080 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature