Allosteric regulation by membranes and hydrophobic subsites in phospholipase A₂ enzymes determine their substrate specificity.

ABSTRACT: Lipids play critical roles in several major chronic diseases of our times, including those that involve inflammatory sequelae such as metabolic syndrome including obesity, insulin sensitivity, and cardiovascular diseases. However, defining the substrate specificity of enzymes of lipid metabolism is a challenging task. For example, phospholipase A₂ (PLA₂) enzymes constitute a superfamily of degradative, biosynthetic, and signaling enzymes that all act stereospecifically to hydrolyze and release the fatty acids of membrane phospholipids. This review focuses on how membranes interact allosterically with enzymes to regulate cell signaling and metabolic pathways leading to inflammation and other diseases. Our group has developed "substrate lipidomics" to quantify the subst

SUBMITTER: Dennis EA 

PROVIDER: S-EPMC9079178 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature