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De novo assembly of Schizothorax waltoni transcriptome to identify immune-related genes and microsatellite markers.


ABSTRACT: Schizothorax waltoni (S. waltoni) is one kind of the subfamily Schizothoracinae and an indigenous economic tetraploid fish to Tibet in China. It is rated as a vulnerable species in the Red List of China's Vertebrates, owing to overexploitation and biological invasion. S. waltoni plays an important role in ecology and local fishery economy, but little information is known about genetic diversity, local adaptation, immune system and so on. Functional gene identification and molecular marker development are the first and essential step for the following biological function and genetics studies. For this purpose, the transcriptome from pooled tissues of three adult S. waltoni was sequenced and analyzed. Using paired-end reads from the Illumina Hiseq4000 platf

SUBMITTER: Ye H 

PROVIDER: S-EPMC9079874 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature