Dataset Information

Label-free impedimetric sensing platform for microRNA-21 based on ZrO₂-reduced graphene oxide nanohybrids coupled with catalytic hairpin assembly amplification.

ABSTRACT: Herein, a sensitive electrochemical impedance sensor was constructed based on ZrO₂-reduced graphene oxide (RGO)-modified electrode coupled with the catalytic hairpin assembly signal amplification strategy. Electrochemical impedance spectroscopy (EIS) was used to detect microRNA (miRNA) using the change in electron transfer resistance (ΔR _et) originated from nucleic acid hybridization on the electrode surface. MiRNA-21 was used as a model to verify this strategy. The results indicated that ΔR _et exhibited a good linear relationship with the concentration of miRNA-21 in the range from 1.0 × 10^-14 mol L^-1 to 1.0 × 10^-10 mol L^-1 with a detection limit of 4.3 × 10^-15 mol L^-1 (S/N = 3). Additionally, this sensor exhibited good selectivity, and it could be applied to detect miRNA-21 in human serum samples and measure the expression levels of miRNA-21 in human breast cancer cell lines (MCF-7); thus, this sensor has great potential in cancer diagnosis.

SUBMITTER: Zhang K

PROVIDER: S-EPMC9080249 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Label-free impedimetric sensing platform for microRNA-21 based on ZrO2-reduced graphene oxide nanohybrids coupled with catalytic hairpin assembly amplification.

Zhang Keying K Zhang Na N Zhang Li L Wang Hongyan H Shi Hongwei H Liu Qiao Q

RSC advances 20180430 29

Herein, a sensitive electrochemical impedance sensor was constructed based on ZrO2-reduced graphene oxide (RGO)-modified electrode coupled with the catalytic hairpin assembly signal amplification strategy. Electrochemical impedance spectroscopy (EIS) was used to detect microRNA (miRNA) using the change in electron transfer resistance (ΔR et) originated from nucleic acid hybridization on the electrode surface. MiRNA-21 was used as a model to verify this strategy. The ...[more]

PMID: 35542230

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Dataset Information

Label-free impedimetric sensing platform for microRNA-21 based on ZrO₂-reduced graphene oxide nanohybrids coupled with catalytic hairpin assembly amplification.

Publications

Label-free impedimetric sensing platform for microRNA-21 based on ZrO<sub>2</sub>-reduced graphene oxide nanohybrids coupled with catalytic hairpin assembly amplification.

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