Dual-cycle immobilization to reuse both enzyme and support by reblossoming enzyme-inorganic hybrid nanoflowers.

ABSTRACT: To achieve dual-reuse of both enzyme and support in enzyme immobilization, hybrid nanoflowers (hNFs) were synthesized and crystallized in aqueous solution using calcium phosphate as inorganic component and enzyme as organic component. When hNFs lost their catalytic activity after reuse for times, they underwent dissolution and recrystallization to achieve the dual-cycle of enzyme and support. Six enzymes including papain, bromelain, trypsin, Lipase from Porcine Pancreas (PPL), Lipase from Thermomyces lanuginosus (TLL) and Lipase B from Candida antarctica (CALB) were chose as model enzymes and the obtained hNFs all presented high catalytic activity and thermal stability. The highest catalytic efficiency (K _cat/K _m) of TLL-hNFs was 38.52 mM

SUBMITTER: Yu J 

PROVIDER: S-EPMC9080259 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature