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Dilute acid catalyzed fractionation and sugar production from bamboo shoot shell in γ-valerolactone/water medium.

ABSTRACT: Overcoming the recalcitrance barrier of cellulosic biomass for efficient production of fermentable sugars at low cost is the current limitation for the industrialization of lignocellulosic biorefineries. In the present work, a two-step non-enzymatic strategy was developed for the fractionation of the main components in bamboo shoot shell (BSS) and conversion of polysaccharides into fermentable sugars by dilute acid in a γ-valerolactone (GVL)/H₂O solvent system. About 86.0% of lignin and 87.4% of hemicelluloses were removed in the first step by 0.6% H₂SO₄ under 140 °C for 1 h with the addition of 60% GVL. The residue solids enriched with cellulose were then subjected to acid hydrolysis employing 0.05% H₂SO₄ as the catalyst in 80% GVL at 180 °C for 20 min. The maximum total soluble sugar yield achieved in the acid hydrolysate was 70.7%. This research could provide valuable insights into the valorization of lignocellulosic biomass and become a promising alternative to the biomass-derived carbohydrate production scheme.

SUBMITTER: Qing Q

PROVIDER: S-EPMC9080427 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Dilute acid catalyzed fractionation and sugar production from bamboo shoot shell in γ-valerolactone/water medium.

Qing Qing Q Gao Xiaohang X Wang Pengbo P Guo Qi Q Xu Zhong Z Wang Liqun L

RSC advances 20180514 31

Overcoming the recalcitrance barrier of cellulosic biomass for efficient production of fermentable sugars at low cost is the current limitation for the industrialization of lignocellulosic biorefineries. In the present work, a two-step non-enzymatic strategy was developed for the fractionation of the main components in bamboo shoot shell (BSS) and conversion of polysaccharides into fermentable sugars by dilute acid in a γ-valerolactone (GVL)/H<sub>2</sub>O solvent system. About 86.0% of lignin a ...[more]

PMID: 35539230

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Project description:BACKGROUND:Gamma valerolactone (GVL) treatment of lignocellulosic bomass is a promising technology for degradation of biomass for biofuel production; however, GVL is toxic to fermentative microbes. Using a combination of chemical genomics with the yeast (Saccharomyces cerevisiae) deletion collection to identify sensitive and resistant mutants, and chemical proteomics to monitor protein abundance in the presence of GVL, we sought to understand the mechanism toxicity and resistance to GVL with the goal of engineering a GVL-tolerant, xylose-fermenting yeast. RESULTS:Chemical genomic profiling of GVL predicted that this chemical affects membranes and membrane-bound processes. We show that GVL causes rapid, dose-dependent cell permeability, and is synergistic with ethanol. Chemical genomic profiling of GVL revealed that deletion of the functionally related enzymes Pad1p and Fdc1p, which act together to decarboxylate cinnamic acid and its derivatives to vinyl forms, increases yeast tolerance to GVL. Further, overexpression of Pad1p sensitizes cells to GVL toxicity. To improve GVL tolerance, we deleted PAD1 and FDC1 in a xylose-fermenting yeast strain. The modified strain exhibited increased anaerobic growth, sugar utilization, and ethanol production in synthetic hydrolysate with 1.5% GVL, and under other conditions. Chemical proteomic profiling of the engineered strain revealed that enzymes involved in ergosterol biosynthesis were more abundant in the presence of GVL compared to the background strain. The engineered GVL strain contained greater amounts of ergosterol than the background strain. CONCLUSIONS:We found that GVL exerts toxicity to yeast by compromising cellular membranes, and that this toxicity is synergistic with ethanol. Deletion of PAD1 and FDC1 conferred GVL resistance to a xylose-fermenting yeast strain by increasing ergosterol accumulation in aerobically grown cells. The GVL-tolerant strain fermented sugars in the presence of GVL levels that were inhibitory to the unmodified strain. This strain represents a xylose fermenting yeast specifically tailored to GVL produced hydrolysates.

Dataset Information

Dilute acid catalyzed fractionation and sugar production from bamboo shoot shell in γ-valerolactone/water medium.

Publications

Dilute acid catalyzed fractionation and sugar production from bamboo shoot shell in γ-valerolactone/water medium.

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