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ITRAQ-based quantitative proteomic analysis of dark-germinated soybeans in response to salt stress.


ABSTRACT: Soybean germination under stressful conditions, especially salt stress, has been verified to be an effective way of accumulating gamma-aminobutyric acid (GABA) in dark-germinated soybeans. In this study, a combination of physiological characteristics and isobaric tags for relative and absolute quantitation (iTRAQ) in a proteomic-based approach was used to investigate the protein changes in dark-germinated soybeans under salt stress. A total of 201 differentially abundant proteins (DAPs) were identified and divided into 13 functional groups. Under salt stress, 20 metabolic pathways were significantly enriched in dark-germinated soybeans. GABA content and antioxidase activity were increased while the growth and development of soybeans were inhibited by the salt stress. Promoting the synthesi

SUBMITTER: Yin Y 

PROVIDER: S-EPMC9080483 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature