Helix loop-mediated isothermal amplification of nucleic acids.

ABSTRACT: Isothermal nucleic acid amplification has played a key role in the point of care test (POCT). In this study, a helix loop-mediated isothermal amplification (HAMP) method with high specificity, efficiency and rapidity was developed. The MERS-Cov orf1b gene was chosen for the validation and optimization of HAMP. The HAMP analysis was performed at a constant temperature of 61-65 °C and yielded a self-primed spiral structure with no introduction of exogenous gene sequence by two pairs of specially designed primers. The primers for helix loop formation were composed of two complementary primers including the helix forward primer and the helix reverse primer, the 3' ends of which were complementary to their respective target nucleic acids. HAMP assay can be monitored by fluorescence signals with

SUBMITTER: Mao R 

PROVIDER: S-EPMC9080610 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature