Exponential magnetophoretic gradient for the direct isolation of basophils from whole blood in a microfluidic system.

ABSTRACT: Despite their rarity in peripheral blood, basophils play important roles in allergic disorders and other diseases including sepsis and COVID-19. Existing basophil isolation methods require many manual steps and suffer from significant variability in purity and recovery. We report an integrated basophil isolation device (i-BID) in microfluidics for negative immunomagnetic selection of basophils directly from 100 μL of whole blood within 10 minutes. We use a simulation-driven pipeline to design a magnetic separation module to apply an exponentially increasing magnetic force to capture magnetically tagged non-basophils flowing through a microtubing sandwiched between magnetic flux concentrators sweeping across a Halbach array. The exponential profile captures non-basophils effectively while p

SUBMITTER: Castano N 

PROVIDER: S-EPMC9080715 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature