Utility of the chromogenic and fluorogenic properties of benzofurazan for the assay of milnacipran in human urine and plasma.

ABSTRACT: Our article presents the development and validation of two simple, very sensitive, and low-cost spectroscopic methods for the assay of milnacipran hydrochloride in bulk form, pharmaceutical tablets and spiked human urine and plasma. Spectroscopic methods (spectrophotometric and spectrofluorimetric techniques) were dependent on the chromogenic and fluorogenic properties of the 4-chloro-7 nitrobenzofurazan (NBD-Cl) reagent. The reaction product, resulting from the interaction between NBD-Cl and milnacipran in the presence of borate buffer pH 8.5, was measured spectrophotometrically at 465 nm and spectrofluorimetrically at 510 nm after excitation at 465 nm. The absorbance-concentration plot was rectilinear over the range of 1.5-12 μg mL^-1 with a limit of quantitation 1.09 μg mL

SUBMITTER: Mostafa IM 

PROVIDER: S-EPMC9081200 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature