Application of Blood Group Genotyping by Next-Generation Sequencing in Various Immunohaematology Cases.

ABSTRACT:

Background

Next-generation sequencing (NGS) technology has been recently introduced into blood group genotyping; however, there are few studies using NGS-based blood group genotyping in real-world clinical settings. In this study, we applied NGS-based blood group genotyping into various immunohaematology cases encountered in routine clinical practice.

Methods

This study included 4 immunohaematology cases: ABO subgroup, ABO chimerism, antibody to a high-frequency antigen (HFA), and anti-CD47 interference. We designed a hybridization capture-based NGS panel targeting 39 blood group-related genes and applied it to the 4 cases.

Results

NGS analysis revealed a novel intronic variant (NM_020469.3:c.29-10T>G) in a patient with an A_el phenotype and detected a small

SUBMITTER: Kim TY 

PROVIDER: S-EPMC9082207 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature