A network of Gα_i signaling partners is revealed by proximity labeling proteomics analysis and includes PDZ-RhoGEF.

ABSTRACT: G protein–coupled receptors (GPCRs) that couple to the Gα_i family of G proteins are key regulators of cell and tissue physiology. Our previous work has revealed new roles for Gα_i in regulating the migration of neutrophils and fibrosarcoma cells downstream of activated chemoattractant receptors. Here, we used an intact cell proximity–based labeling coupled to tandem mass tag (TMT)–based quantitative proteomics analysis to identify proteins that selectively interacted with the GTP-bound form of Gα_i1. Multiple targets were identified and validated with a BioID2-tagged, constitutively active Gα_i1 mutant, suggesting a network of interactions for activated Gα_I proteins in intact cells. We showed that active Gα_i1, but not Gα_i2

SUBMITTER: Chandan NR 

PROVIDER: S-EPMC9082328 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature