Hot isopropanol quenching procedure for automated microtiter plate scale ¹³C-labeling experiments.

ABSTRACT:

Background

Currently, the generation of genetic diversity for microbial cell factories outpaces the screening of strain variants with omics-based phenotyping methods. Especially isotopic labeling experiments, which constitute techniques aimed at elucidating cellular phenotypes and supporting rational strain design by growing microorganisms on substrates enriched with heavy isotopes, suffer from comparably low throughput and the high cost of labeled substrates.

Results

We present a miniaturized, parallelized, and automated approach to ¹³C-isotopic labeling experiments by establishing and validating a hot isopropanol quenching method on a robotic platform coupled with a microbioreactor cultivation system. This allows for the first time to conduct automated labeling

SUBMITTER: Nießer J 

PROVIDER: S-EPMC9082905 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature