Decorating a single giant DNA with gold nanoparticles.

ABSTRACT: We decorated a single giant DNA (1.66 × 10⁵ base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy revealed that individual decorated DNA molecules stay in the bulk solution by avoiding aggregation and precipitation, and exhibit translational and conformational fluctuation, i.e., Brownian motion. An analysis of the intra-chain fluctuation of single DNA molecules revealed that the apparent spring constant and damping coefficient of a DNA chain increased by ca. 13- and 5-fold, respectively, upon decoration with gold nanoparticles. Observation by transmission electron microscopy revealed that gold nanoparticles were stably attached to the DNA s

SUBMITTER: Carnerero JM 

PROVIDER: S-EPMC9083275 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature