Deep Metabolic Profiling Assessment of Tissue Extraction Protocols for Three Model Organisms.

ABSTRACT: Metabolic profiling harbors the potential to better understand various disease entities such as cancer, diabetes, Alzheimer's, Parkinson's disease or COVID-19. To better understand such diseases and their intricate metabolic pathways in human studies, model animals are regularly used. There, standardized rearing conditions and uniform sampling strategies are prerequisites towards a successful metabolomic study that can be achieved through model organisms. Although metabolomic approaches have been employed on model organisms before, no systematic assessment of different conditions to optimize metabolite extraction across several organisms and sample types has been conducted. We address this issue using a highly standardized metabolic profiling assay analyzing 630 metabolites across three co

SUBMITTER: Gegner HM 

PROVIDER: S-EPMC9083328 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature