AtRsmD Is Required for Chloroplast Development and Chloroplast Function in Arabidopsis thaliana.

ABSTRACT: AtRsmD was recently demonstrated to be a chloroplast 16S rRNA methyltransferase (MTase) for the m²G915 modification in Arabidopsis. Here, its function of AtRsmD for chloroplast development and photosynthesis was further analyzed. The AtRsmD gene is highly expressed in green photosynthetic tissues. AtRsmD is associated with the thylakoid in chloroplasts. The atrsmd-2 mutant exhibited impaired photosynthetic efficiency in emerging leaves under normal growth conditions. A few thylakoid lamellas could be observed in the chloroplast from the atrsmd-2 mutant, and these thylakoids were loosely organized. Knockout of the AtRsmD gene had minor effects on chloroplast ribosome biogenesis and RNA loading on chloroplast ribosomes, but it reduced the amounts

SUBMITTER: Wang ZY 

PROVIDER: S-EPMC9083416 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature