Incorporation of antimicrobial peptides on electrospun nanofibres for biomedical applications.

ABSTRACT: The aim of this work was to immobilize antimicrobial peptides onto a fibrous scaffold to create functional wound dressings. The scaffold was produced by electrospinning from a mixture of the water soluble polymers poly(acrylic acid) and poly(vinyl alcohol) and subsequently heat cured at 140 °C to produce a stable material with fibre diameter below micron size. The peptides were incorporated into the negatively charged scaffold by electrostatic interaction. The best results were obtained for lysozyme impregnated at pH 7, which rendered a loading of up to 3.0 × 10^-4 mmol mg^-1. The dressings were characterized using SEM, ATR-FTIR, elemental analysis, ζ-potential and confocal microscopy using fluorescamine as an amine-reactive probe. The dressings preserved their fibrous

SUBMITTER: Amariei G 

PROVIDER: S-EPMC9083935 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature