Analysis of combinatorial CRISPR screens with the Orthrus scoring pipeline.

ABSTRACT: The continued improvement of combinatorial CRISPR screening platforms necessitates the development of new computational pipelines for scoring combinatorial screening data. Unlike for single-guide RNA (sgRNA) pooled screening platforms, combinatorial scoring for multiplexed systems is confounded by guide design parameters such as the number of gRNAs per construct, the position of gRNAs along constructs, and additional features that may impact gRNA expression, processing or capture. In this protocol we describe Orthrus, an R package for processing, scoring and analyzing combinatorial CRISPR screening data that addresses these challenges. This protocol walks through the application of Orthrus to previously published combinatorial screening data from the CHyMErA experimental system, a platform

SUBMITTER: Ward HN 

PROVIDER: S-EPMC9084619 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature